was calculated from the value of m/z.B MS/MS analysis of a precursor ion of m/z 1286.942+. Fragment ions m/z 689+ corresponding to -P-Man-(EthN-P-)Man-GlcN, m/z 949+ corresponding to P-Man-(EthN-P-)Man-GlcN-Ino-P, m/z 1152+ corresponding to P-Man-(EthN-P-)Man-GlcN-Ino-P-HexNAc and m/z 1948+ corresponding to (11 amino acids)-EthN-P-Man-(EthN-P-)Man, indicate CD59 C-terminus attachment to EthN-P linked to the second mannose.A novel structure of GPI anchored proteins and its physiological roleYoshiko MurakamiFigure 2. Mass spectrometric analysis of trypsin fragments of CD59 expressed in PIGB-KO HEK293 cellsA Schematic MS/MS profile showing the CD59 C-terminal eleven amino acids linked to a GPI glycan with only two mannoses. M.W. Figure 3. Schematic illustration of the novel structure within the GPI biosynthesis pathway Most GPI precursor proteins attach to the EthN-P on the third mannose while some, such as NT5E and NTNG2 have preference to attach to the EthN-P on the second mannose.96
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